Immobilization of a Commercial Lipase from Penicillium camembertii (Lipase G) by Different Strategies

Figure 1

Immobilization course for Lipase G on MANAE-agarose by ionic adsorption (a) and covalent attachment (b) on MANAE-agarose-glutaraldehyde incubated in 100 mmoL·L⁻¹ buffer phosphate pH 7.0 (open circle), 100 mmoL·L⁻¹ buffer Tris-HCl pH 7.0 (open triangle), and 2.5 mmoL·L⁻¹ buffer Tris-HCl pH 7.0 (full triangle) and supernatant (full square).

(a)

(b)