Molecular Cloning and Characterization of P4 Nuclease from Leishmania infantum
Figure 7
Analysis of P4 gene expression in different life cycle stages of L. infantum. (a) Promastigote and amastigote cell lysates were analyzed by immunoblotting using rabbit anti-recombinant Li-P4. Lane 1: Log phase promastigotes. Lane 2: Stationary phase promastigotes. Lane 3: Amastigotes. (b) The same blot as in a was probed with rabbit anti Elongation Factor 1-alfa antibody. The results indicate higher expression of P4 nuclease in amastigote stage of parasite.
