The Role of Arg13 in Protein Phosphatase M tPphA from Thermosynechococcus elongatus

Table 1

Primers used for PCR amplification of tPphA and for site-directed mutagenesis.

T7	5′; 5′-TAATACGACTCACTATAGGG-3′
	3′; 5′-GCTAGTTATTGCTCAGCGG-3′
R13X	5′; 5′-CTGACTGTGGTCTGATTNNNAAAAGCAATCAGGATGC-3′
	3′; 5′-GCATCCTGATTGCTTTTNNNAATCAGACCACAGTCAG-3′
R13A	5′; 5′-CTGACTGTGGTCTGATTGCTAAAAGCAATCAGGATGC-3′
	3′; 5′-GCATCCTGATTGCTTTTAGCAATCAGACCACAGTCAG-3′
R13C	5′; 5′-CTGACTGTGGTCTGATTTGTAAAAGCAATCAGGATGC-3′
	3′; 5′-GCATCCTGATTGCTTTTACAAATCAGACCACAGTCAG-3′
R13E	5′; 5′-CTGACTGTGGTCTGATTGAAAAAAGCAATCAGGATGC-3′
	3′; 5′-GCATCCTGATTGCTTTTTTCAATCAGACCACAGTCAG-3′
R13V	5′; 5′-CTGACTGTGGTCTGATTGTTAAAAGCAATCAGGATGC-3′
	3′; 5′-GCATCCTGATTGCTTTTAACAATCAGACCACAGTCAG-3′