Research Article

Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

Figure 5

Contour diagrams for (a) free lipase and (b) immobilized lipase activities as a function of pH and temperature according to the second and third experimental designs. Optimal temperature and pH were determined in buffer solutions (pH 3.0 to 9.0) at different temperatures (30–70°C).