Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology
Table 1
Coded levels and real values (in parentheses) for the first factorial design (20 trials) and immobilized lipase activity at 25°C.
Run
pH**
t (min)
ES* (U/g-support)
IE* (U/g-support)
1
−1 (3)
−1 (60)
−1 (405)
2
2
+1 (7)
−1 (60)
−1 (405)
5
3
−1 (3)
+1 (240)
−1 (405)
24
4
+1 (7)
+1 (240)
−1 (405)
6
5
−1 (3)
−1 (60)
+1 (1600)
27
6
+1 (7)
−1 (60)
+1 (1600)
30
7
−1 (3)
+1 (240)
+1 (1600)
153
8
+1 (7)
+1 (240)
+1 (1600)
32
9
−1.68 (1)
0 (150)
0 (1000)
1
10
+1.68 (9)
0 (150)
0 (1000)
1
11
0 (5)
−1.68 (1)
0 (1000)
179
12
0 (5)
+1.68 (300)
0 (1000)
101
13
0 (5)
0 (150)
−1.68 (1)
1
14
0 (5)
0 (150)
+1.68 (1999)
163
15
0 (5)
0 (150)
0 (1000)
168
16
0 (5)
0 (150)
0 (1000)
165
17
0 (5)
0 (150)
0 (1000)
173
18
0 (5)
0 (150)
0 (1000)
166
19
0 (5)
0 (150)
0 (1000)
167
20
0 (5)
0 (150)
0 (1000)
171
*ES: enzyme/support ratio and IE: immobilized enzyme.
**Buffer solutions:1 M HCl for pH 1.0; 0.05 M citrate-phosphate buffer for pH 2.0, 3.0, 4.0, 5.0, 6.0, and 7.0; 0.05 M Tris-HCl buffer for pH 8.0 and 9.0.
