Enzyme Research

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Research Article

Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

Table 1

Coded levels and real values (in parentheses) for the first factorial design (20 trials) and immobilized lipase activity at 25°C.

RunpH**t (min)ES* (U/g-support)IE* (U/g-support)

1−1 (3)−1 (60)−1 (405)2
2+1 (7)−1 (60)−1 (405)5
3−1 (3)+1 (240)−1 (405)24
4+1 (7)+1 (240)−1 (405)6
5−1 (3)−1 (60)+1 (1600)27
6+1 (7)−1 (60)+1 (1600)30
7−1 (3)+1 (240)+1 (1600)153
8+1 (7)+1 (240)+1 (1600)32
9−1.68 (1)0 (150)0 (1000)1
10+1.68 (9)0 (150)0 (1000)1
110 (5)−1.68 (1)0 (1000)179
120 (5)+1.68 (300)0 (1000)101
130 (5)0 (150)−1.68 (1)1
140 (5)0 (150)+1.68 (1999)163
150 (5)0 (150)0 (1000)168
160 (5)0 (150)0 (1000)165
170 (5)0 (150)0 (1000)173
180 (5)0 (150)0 (1000)166
190 (5)0 (150)0 (1000)167
200 (5)0 (150)0 (1000)171

*ES: enzyme/support ratio and IE: immobilized enzyme.
**Buffer solutions:1 M HCl for pH 1.0; 0.05 M citrate-phosphate buffer for pH 2.0, 3.0, 4.0, 5.0, 6.0, and 7.0; 0.05 M Tris-HCl buffer for pH 8.0 and 9.0.

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