Enzyme Research

Enzyme Research / 2012 / Article / Tab 2

Research Article

Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

Table 2

Coded levels and real values (in parentheses) for the second (12 trials) and third (13 trials) factorial design for free and immobilized lipase activity.

RunpH**T (°C)FLCS* (U/mL supernatant)IE* (U/g-support)

1−1 (4)−1 (36)289
2+1 (8)−1 (36)2011
3−1 (4)+1 (64)12812
4+1 (8)+1 (64)1313
5−1.41 (3)0 (50)7912
6+1.41(9)0 (50)035
70 (6)−1.41 (30)379
80 (6)+1.41 (70)14551
90 (6)0 (50)11974
100 (6)0 (50)12262
110 (6)0 (50)11974
120 (6)0 (50)12369
130 (6)0 (50)69

*FLCS: free enzyme and IE: immobilized enzyme.
**Buffer solutions: 0.05 M citrate-phosphate buffer for pH 3.0, 4.0, 5.0, 6.0, and 7.0; 0.05 M Tris-HCl buffer for pH 8.0 and 9.0.

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