Simulation of Enzyme Catalysis in Calcium Alginate Beads

Table 1

𝐾_𝑚,𝑉_max, and 𝑘₂ values of starch hydrolysis using immobilized amylase enzyme in fixed bed reactor at different values of initial substrate concentration and residence time (pH = 5.5, 𝑇 = 50°C).

[S] g/L	𝐾𝑚 (g/L)
	Residence time, min
	5	10	20	40	60	90	120
1	0.340	0.428	0.510	0.658	0.790	0.926	1.010
5	1.690	2.137	2.550	3.290	3.940	4.630	5.060
10	3.380	4.270	5.100	6.580	7.870	9.260	10.13
50	16.89	21.37	25.51	32.90	39.37	46.30	50.66
100	33.78	42.74	51.00	65.79	78.74	92.60	101.3
	𝑉max (g/L·s)
1	0.013	0.009	0.008	0.007	0.006	0.005	0.002
5	0.067	0.047	0.038	0.036	0.181	0.023	0.009
10	0.134	0.094	0.077	0.072	0.362	0.046	0.017
50	0.672	0.470	0.383	0.362	1.811	0.232	0.086
100	1.344	0.940	0.765	0.724	3.622	0.463	0.172
	𝑘2 (s^−1)
1	0.747	0.522	0.425	0.402	0.350	0.257	0.096
5	3.734	2.611	2.126	2.010	10.06	1.286	0.478
10	7.467	5.223	4.252	4.020	20.12	2.572	0.957
50	37.34	26.12	21.26	20.10	100.6	12.86	4.784
100	74.68	52.23	42.52	40.20	201.2	25.72	9.569