Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042

Table 5

Rotatable CCD matrix for five variables with actual protease activity.

Sl. number	𝐴	𝐵	𝐶	𝐷	𝐸	Protease activity (U)
1	2	30	7	120	72	132.49
2	3	30	7	120	72	122.78
3	2	40	7	120	72	157.69
4	3	40	7	120	72	152.79
5	2	30	8	120	72	115.42
6	3	30	8	120	72	102.48
7	2	40	8	120	72	136.70
8	3	40	8	120	72	131.90
9	2	30	7	180	72	233.79
10	3	30	7	180	72	215.84
11	2	40	7	180	72	261.54
12	3	40	7	180	72	249.97
13	2	30	8	180	72	213.10
14	3	30	8	180	72	193.58
15	2	40	8	180	72	239.87
16	3	40	8	180	72	223.89
17	2	30	7	120	96	170.54
18	3	30	7	120	96	152.69
19	2	40	7	120	96	200.84
20	3	40	7	120	96	190.84
21	2	30	8	120	96	134.84
22	3	30	8	120	96	116.60
23	2	40	8	120	96	164.75
24	3	40	8	120	96	151.32
25	2	30	7	180	96	242.52
26	3	30	7	180	96	215.75
27	2	40	7	180	96	276.16
28	3	40	7	180	96	257.23
29	2	30	8	180	96	203.78
30	3	30	8	180	96	177.30
31	2	40	8	180	96	236.54
32	3	40	8	180	96	216.43
33	1.3	35	7.5	150	84	286.35
34	3.7	35	7.5	150	84	250.07
35	2.5	23	7.5	150	84	101.30
36	2.5	47	7.5	150	84	174.75
37	2.5	35	6.3	150	84	160.24
38	2.5	35	8.7	150	84	88.85
39	2.5	35	7.5	79	84	103.75
40	2.5	35	7.5	221	84	300.87
41	2.5	35	7.5	150	55.5	131.02
42	2.5	35	7.5	150	112.5	163.67
43	2.5	35	7.5	150	84	273.31
44	2.5	35	7.5	150	84	274.10
45	2.5	35	7.5	150	84	272.82
46	2.5	35	7.5	150	84	132.49
47	2.5	35	7.5	150	84	122.78
48	2.5	35	7.5	150	84	157.69
49	2.5	35	7.5	150	84	152.79
50	2.5	35	7.5	150	84	115.42