Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042

Table 6

Regression analysis for the production of alkaline protease by Bacillus licheniformis NCIM 2042 for quadratic response surface model fitting (ANOVA).

Source	Sum of squares	Degree of freedom	Mean square	Coefficient 𝑡 estimate	Standard error	𝐹-value	𝑃 value prob > 𝐹
Model*	181834.9	20	9091.75	—	—	5602.23	<0.0001	Significant
Intercept	—	—	—	271.59	0.45	—	<0.0001
𝐴	2598.525	1	2598.53	−7.75	0.19	1601.18	<0.0001
𝐵	10664.44	1	10664.44	15.69	0.19	6571.31	<0.0001
𝐶	9597.122	1	9597.12	−14.89	0.19	5913.64	<0.0001
𝐷	74093.66	1	74093.66	41.36	0.19	45655.68	<0.0001
𝐸	2104.942	1	2104.94	6.97	0.19	1297.04	<0.0001
𝐴𝐵	77.25183	1	77.25	1.55	0.23	47.60	<0.0001
𝐴𝐶	5.974906	1	5.97	−0.43	0.23	3.68	0.0649
𝐴𝐷	133.704	1	133.70	−2.04	0.23	82.39	<0.0001
𝐴𝐸	92.57182	1	92.57	−1.70	0.23	57.04	<0.0001
𝐵𝐶	8.381578	1	8.38	−0.51	0.23	5.16	0.0306
𝐵𝐷	22.72784	1	22.73	0.84	0.23	14.00	0.0008
𝐵𝐸	95.25979	1	95.26	1.73	0.23	58.70	<0.0001
𝐶𝐷	14.67836	1	14.68	−0.68	0.23	9.04	0.0054
𝐶𝐸	569.8502	1	569.85	−4.22	0.23	351.14	<0.0001
𝐷𝐸	1741.185	1	1741.19	−7.38	0.23	1072.90	<0.0001
𝐴^2	19.17653	1	19.18	−0.59	0.17	11.82	0.0018
𝐵^2	30951.91	1	30951.91	−23.60	0.17	19072.22	<0.0001
𝐶^2	37519.93	1	37519.93	−25.98	0.17	23119.36	<0.0001
𝐷^2	8321.356	1	8321.36	−12.24	0.17	5127.53	<0.0001
𝐸^2	26782.87	1	26782.87	−21.95	0.17	16503.30	<0.0001
Residual	47.0635	29	1.62
Lack of Fit	8.692591	22	0.40			0.07	1	Not significant
Pure Error	38.37091	7	5.48
Cor Total	181882	49

*SD: 1.27; Mean: 198.51; R-Squared: 0.9997; Adj R-Squared: 0.9996; C.V.%: 0.64; PRESS: 81.03.