Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042

Table 7

Validation of quadratic model within the design space.

Number	Inoculum percentage %	Temperature °C	pH	Agitation RPM	Incubation time h	Actual response (U)	Predict response (U)
1	2	35	7	120	72	165.24	168.68
2	2.5	37	7.5	150	72	242.03	244.48
3	3	40	8	160	96	199.17	205.25
4	2.5	30	7.8	180	84	139.65	242.2
5	3	37	7.4	150	74	248.21	247.53
6	2.0	36.6	7.35	180	85	315.28	314.65