Review Article
Pullulanase: Role in Starch Hydrolysis and Potential Industrial Applications
Table 2
Reaction specificities of pullulan-degrading enzymes.
| Enzyme | EC number | Bonds processed | Preferred substrate | End products | References |
| Pullulanase type I
| 3.2.1.41 | α-(1,6) | Oligo- and polysaccharides, Pullulan | Trimer (maltotriose) | [17, 27] |
| Pullulanase type II (amylopullulanase) | | α-(1,6) | Pullulan, | Trimer (maltotriose) | |
3.2.1.41 | α-(1,4) | Poly- and oligosaccharide (starch) | Mixture of glucose, maltose, and maltotriose |
[31, 32, 40] |
| Pullulan hydrolase type I (neopullulanase) | 3.2.1.135 | α-(1,4) | Pullulan | Panose | [41–43] |
| Pullulan hydrolase type II (isopullulanase) | 3.2.1.57 | α-(1,4) | Pullulan | Isopanose | [12] |
| Pullulan hydrolase type III | | α-(1,4) and α-(1,6) | Pullulan | Mixture of panose, maltose, and maltotriose | |
3.2.1.— | | Starch, amylose, and amylopectin | Maltotriose and maltose |
[37] |
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