Table 6: Genetic engineering of pullulanase gene.

SourceExpression strainLength (a.a)*VectorReferences

Anaerobranca gottschalkiiE. coli BL21 (DE3)
865pET21a[60]
Bacillus thermoleovorans US105E. coli DH5α718pEBM2[30]
Bacillus stearothermophilus TS-23E. coli XL1-Blue MRF2018pAP/StuI
[61]
Bacillus stearothermophilus strain TRS-128
B. subtilis NA-1N.A.#pTB522[62]
Klebsiella pneumoniaE. coli K-12N.A.#pACYC184[63]
Thermus IM6501E. coli718p6xHis119[64]
Desulfurococcus mucosusB. subtilis clone JA803
686JA803[32]
Fervidobacterium pennivorans Ven5
E. coli FD748849pSE420[29]
Thermococcus hydrothermalisE. coli JM109
(DE)
1339pAPUΔ2[65]
Thermotoga maritimaE. coli JM83840pTPU1[66]
Caldicellulosiruptor saccharolyticusE. coli Q359826pNZ1038[67]
Bacillus sp. XAL601 IIE. coli JM109
2032pUC18[68]

*a.a: amino acid.
#N.A.: not available.