Table of Contents Author Guidelines Submit a Manuscript
Enzyme Research
Volume 2013 (2013), Article ID 287343, 10 pages
http://dx.doi.org/10.1155/2013/287343
Research Article

An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues

1Department of Cellular Biology, Institute of Biological Sciences, University of Brasilia, 70.910-900 Brasilia, DF, Brazil
2Department of Biochemistry and Molecular Biology, Institute of Biological Sciences, Federal University of Goias, 74.001-970 Goiania, GO, Brazil
3Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, ET 18/25, Darcy Ribeiro University Campus, 70.910-900 Brasilia, DF, Brazil

Received 19 March 2013; Revised 11 June 2013; Accepted 13 June 2013

Academic Editor: Joaquim Cabral

Copyright © 2013 Eveline Queiroz de Pinho Tavares et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. A. Lockington, L. Rodbourn, S. Barnett, C. J. Carter, and J. M. Kelly, “Regulation by carbon and nitrogen sources of a family of cellulases in Aspergillus nidulans,” Fungal Genetics and Biology, vol. 37, no. 2, pp. 190–196, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. T. Schneider, B. Gerrits, R. Gassmann et al., “Proteome analysis of fungal and bacterial involvement in leaf litter decomposition,” Proteomics, vol. 10, no. 9, pp. 1819–1830, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. S. Saykhedkar, A. Ray, P. Ayoubi-Canaan, S. D. Hartson, R. Prade, and A. J. Mort, “A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover,” Biotechnology for Biofuels, vol. 5, no. 52, pp. 1–17, 2012. View at Google Scholar
  4. G. Chikamatsu, K. Shirai, M. Kato, T. Kobayashi, and N. Tsukagoshi, “Structure and expression properties of the endo-β-1,4-glucanase A gene from the filamentous fungus Aspergillus nidulans,” FEMS Microbiology Letters, vol. 175, no. 2, pp. 239–245, 1999. View at Publisher · View at Google Scholar · View at Scopus
  5. S. H. Kling, C. Carvalho Neto, M. A. Ferrara, J. C. R. Torres, D. B. Magalhães, and D. D. Y. Ryu, “Enhancement of enzymatic hydrolysis of sugar cane bagasse by steam explosion pretreatment,” Biotechnology and Bioengineering, vol. 29, no. 8, pp. 1035–1039, 1987. View at Google Scholar · View at Scopus
  6. M. R. Rubini, A. J. P. Dillon, C. M. Kyaw, F. P. Faria, M. J. Poças-Fonseca, and I. Silva-Pereira, “Cloning, characterization and heterologous expression of the first Penicillium echinulatum cellulase gene,” Journal of Applied Microbiology, vol. 108, no. 4, pp. 1187–1198, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. J. Sambrook and D. W. Russel, Molecular Cloning—A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, NY, USA, 3rd edition, 2001.
  8. H. Blum, H. Beier, and H. J. Gross, “Improved silver staining of plant proteins, RNA and DNA in polyacrilamide gels,” Electrophoresis, vol. 8, no. 2, pp. 93–99, 1987. View at Google Scholar
  9. X. Sun, Z. Liu, Y. Qu, and X. Li, “The effects of wheat bran composition on the production of biomass-hydrolyzing enzymes by Penicillium decumbens,” Applied Biochemistry and Biotechnology, vol. 146, no. 1–3, pp. 119–128, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. M. Mandels, R. Andreotti, and C. Roche, “Measurement of saccharifying cellulase,” Biotechnology and Bioengineering Symposium, no. 6, pp. 21–33, 1976. View at Google Scholar · View at Scopus
  11. E. X. F. Filho, J. Puls, and M. P. Coughlan, “Biochemical characteristics of two endo-β-1,4-xylanases produced by Penicillium capsulatum,” Journal of Industrial Microbiology, vol. 11, no. 3, pp. 171–180, 1993. View at Google Scholar · View at Scopus
  12. G. L. Miller, “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, no. 3, pp. 426–428, 1959. View at Google Scholar · View at Scopus
  13. A. P. de Souza, D. C. C. Leite, S. Pattathil, M. G. Hahn, and M. S. Buckeridge, “Composition and structure of sugarcane cell wall polysaccharides: implications for second-generation bioethanol production,” BioEnergy Research, vol. 6, no. 2, pp. 564–579, 2013. View at Google Scholar
  14. S. E. Chaabouni, T. Mechichi, F. Limam, and N. Marzouki, “Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant Pol 6,” Applied Biochemistry and Biotechnology, vol. 125, no. 2, pp. 99–112, 2005. View at Google Scholar · View at Scopus
  15. O. Ribeiro, M. Wiebe, M. Ilmén, L. Domingues, and M. Penttilä, “Expression of Trichoderma reesei cellulases CBHI and EGI in Ashbya gossypii,” Applied Microbiology and Biotechnology, vol. 87, no. 4, pp. 1437–1446, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. J. Thongekkaew, H. Ikeda, K. Masaki, and H. Iefuji, “An acidic and thermostable carboxymethyl cellulase from the yeast Cryptococcus sp. S-2: purification, characterization and improvement of its recombinant enzyme production by high cell-density fermentation of Pichia pastoris,” Protein Expression and Purification, vol. 60, no. 2, pp. 140–146, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. Y. Bai, R. Guo, H. Yu, L. Jiao, S. Ding, and Y. Jia, “Cloning of endo-β-glucanase I gene and expression in Pichia pastoris,” Frontiers of Agriculture in China, vol. 5, no. 2, pp. 196–200, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. G. S. Oliveira, C. J. Ulhoa, M. H. L. Silveira et al., “An alkaline thermostable recombinant Humicola grisea var. thermoidea cellobiohydrolase presents bifunctional (endo/exoglucanase) activity on cellulosic substrates,” World Journal of Microbiology and Biotechnology, vol. 29, no. 1, pp. 19–26, 2013. View at Publisher · View at Google Scholar
  19. S. K. Garg and S. Neelakantan, “Effect of cultural factors on cellulase activity and protein production by Aspergillus terreus,” Biotechnology and Bioengineering, vol. 24, no. 1, pp. 109–125, 1982. View at Google Scholar
  20. J. Gao, H. Weng, Y. Xi, D. Zhu, and S. Han, “Purification and characterization of a novel endo-β-1,4-glucanase from the thermoacidophilic Aspergillus terreus,” Biotechnology Letters, vol. 30, no. 2, pp. 323–327, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. B. Hahn-Hägerdal, M. Galbe, M. F. Gorwa-Grauslund, G. Lidén, and G. Zacchi, “Bio-ethanol—the fuel of tomorrow from the residues of today,” Trends in Biotechnology, vol. 24, no. 12, pp. 549–556, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Dashtban, H. Schraft, and W. Qin, “Fungal bioconversion of lignocellulosic residues; Opportunities & perspectives,” International Journal of Biological Sciences, vol. 5, no. 6, pp. 578–595, 2009. View at Google Scholar · View at Scopus
  23. A. M. Elshafei, M. M. Hassan, B. M. Haroun, O. M. Abdel-Fatah, H. M. Atta, and A. M. Othman, “Purification and properties of an endoglucanase of Aspergillus terreus DSM 826,” Journal of Basic Microbiology, vol. 49, no. 5, pp. 426–432, 2009. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Karnchanatat, A. Petsom, P. Sangvanich et al., “A novel thermostable endoglucanase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm,” Enzyme and Microbial Technology, vol. 42, no. 5, pp. 404–413, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. S.-Y. Liu, M. A. Shibu, H.-J. Jhan, C.-T. Lo, and K.-C. Peng, “Purification and characterization of novel glucanases from Trichoderma harzianum ETS 323,” Journal of Agricultural and Food Chemistry, vol. 58, no. 19, pp. 10309–10314, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. D. Liu, R. Zhang, X. Yang et al., “Expression, purification and characterization of two thermostable endoglucanases cloned from a lignocellulosic decomposing fungi Aspergillus fumigatus Z5 isolated from compost,” Protein Expression and Purification, vol. 79, no. 2, pp. 176–186, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. A. Nazir, R. Soni, H. S. Saini, R. K. Manhas, and B. S. Chadha, “Purification and characterization of an endoglucanase from Aspergillus terreus highly active against barley β-glucan and xyloglucan,” World Journal of Microbiology and Biotechnology, vol. 25, no. 7, pp. 1189–1197, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. J. S. van Dyk and B. I. Pletschke, “A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes—factors affecting enzymes, conversion and synergy,” Biotechnology Advances, vol. 30, pp. 1458–1480, 2012. View at Publisher · View at Google Scholar · View at Scopus
  29. L. R. Lynd, P. J. Weimer, W. H. van Zyl, and I. S. Pretorius, “Microbial cellulose utilization: fundamentals and biotechnology,” Microbiology and Molecular Biology Reviews, vol. 66, no. 3, pp. 506–577, 2002. View at Publisher · View at Google Scholar · View at Scopus
  30. D. Mattanovich, A. Graf, J. Stadlmann et al., “Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris,” Microbial Cell Factories, vol. 8, article 29, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. A. Salinas, M. Vega, M. E. Lienqueo, A. Garcia, R. Carmona, and O. Salazar, “Cloning of novel cellulases from cellulolytic fungi: heterologous expression of a family 5 glycoside hydrolase from Trametes versicolor in Pichia pastoris,” Enzyme and Microbial Technology, vol. 49, no. 6-7, pp. 485–491, 2011. View at Publisher · View at Google Scholar · View at Scopus
  32. R. A. Nieves, C. I. Ehrman, W. S. Adney, R. T. Elander, and M. E. Himmel, “Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol,” World Journal of Microbiology and Biotechnology, vol. 14, no. 2, pp. 301–304, 1998. View at Publisher · View at Google Scholar · View at Scopus
  33. C. Sánchez, “Lignocellulosic residues: biodegradation and bioconversion by fungi,” Biotechnology Advances, vol. 27, no. 2, pp. 185–194, 2009. View at Publisher · View at Google Scholar · View at Scopus
  34. M. G. Adsul, J. E. Ghule, R. Singh et al., “Polysaccharides from bagasse: applications in cellulase and xylanase production,” Carbohydrate Polymers, vol. 57, no. 1, pp. 67–72, 2004. View at Publisher · View at Google Scholar · View at Scopus
  35. M. G. Adsul, J. E. Ghule, H. Shaikh et al., “Enzymatic hydrolysis of delignified bagasse polysaccharides,” Carbohydrate Polymers, vol. 62, no. 1, pp. 6–10, 2005. View at Publisher · View at Google Scholar · View at Scopus
  36. S. D. Mansfield, C. Mooney, and J. N. Saddler, “Substrate and enzyme characteristics that limit cellulose hydrolysis,” Biotechnology Progress, vol. 15, no. 5, pp. 804–816, 1999. View at Publisher · View at Google Scholar · View at Scopus
  37. R. L. Howard, E. Abotsi, E. L. J. van Rensburg, and S. Howard, “Lignocellulose biotechnology: issues of bioconversion and enzyme production,” African Journal of Biotechnology, vol. 2, no. 12, pp. 602–619, 2003. View at Google Scholar