Table 1: Purification of the recombinant endoglucanase from the supernatant of P. pastoris.

FractionsTotal protein (µg)Recombinant endoglucanase A activity
Total activity (IU)Specific activity (IU/mg)Yield (%)Purification fold (x)

Crude extract36.69116.943.21001
Concentrated fraction (MW50)19.979.450.5NDND
Ultrafiltered fraction (MW50)21.3577.043.665.91.1
Concentrated fraction (MW10)12.513.950.33.40.1
Ultrafiltered fraction (MW10)24.6689.573.6NDND
Sephadex G500.314.3314.13.74.4
Dialyzed0.364.1211.53.53.6
Q-Sepharose0.042.4055.82.117.5

ND: not determined.