Research Article

An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues

Table 2

Effect of treatment with different agents (chelators, metal ions, detergents, and reducing agents) on rEG A activity.

TreatmentRelative activity (%)

Control100.00 ± 3.95
SDS31.24 ± 4.73*
EDTA103.69 ± 4.07
DTT132.43 ± 3.11*
FeCl3 6H2O92.00 ± 6.09
AlCl367.10 ± 4.54*
CaCl2112.55 ± 6.91*
ZnSO483.03 ± 7.19*
NaCl97.14 ± 4.30
CoCl2 7H2O132.26 ± 3.19*
CuCl2120.37 ± 2.33*
KCl99.34 ± 4.20
-mercaptoethanol181.81 ± 11.09*

Asterisks (*) indicate statistical difference within the same fraction ( ) when compared to control. The results are presented in terms of activity ± standard deviation. The endoglucanase activity was assayed after the addition of 18 mM of the agents to the reaction system.