Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation

Table 1

Central composite design with the experimental, predicted responses and its 𝑅-studentized residuals.

Run	Input parameters					Response, La (U/mL)		𝑅-studentized residual
	Temp.^a (°C)	OC^b (%)	IS^c (%)	pH	IT^d (h)	Exp.	Predict.
	(𝑋1)	(𝑋2)	(𝑋3)	(𝑋4)	(𝑋5)
1	35	12	10	8	4	3.06	2.973	0.479
2	30	14	12	7	4	4.62	4.708	−2.195
3	30	10	12	9	4	3.86	3.862	−0.042
4	30	10	8	7	4	3.74	3.761	−0.437
5	30	14	12	9	2	5.68	5.722	−0.884
6	35	12	10	8	3	3.26	3.528	−1.111
7	35	10	10	8	3	4.92	4.978	−0.318
8	40	14	8	9	2	4.87	4.843	0.561
9	40	14	8	7	4	3.91	3.929	−0.402
10	35	12	10	8	3	3.27	3.522	−1.064
11	35	12	8	8	3	2.90	2.923	−0.128
12	40	10	8	9	4	5.38	5.313	1.509
13	30	12	10	8	3	3.71	3.458	1.533
14	40	10	12	9	2	4.33	4.294	0.748
15	35	12	10	8	3	3.26	3.522	−1.111
16	35	12	10	8	2	3.86	3.522	1.491
17	30	10	8	9	3	4.78	4.754	0.525
18	35	12	10	9	2	4.42	4.520	−0.556
19	30	14	8	7	3	3.16	3.221	−1.351
20	40	12	8	7	2	3.37	3.434	−0.355
21	30	10	10	8	4	3.39	3.442	−1.338
22	40	10	12	7	3	3.19	3.201	−0.224
23	35	14	12	7	4	5.46	5.214	1.484
24	40	14	10	8	2	4.70	4.700	−0.009
25	40	14	12	9	3	3.15	3.200	−1.093
26	35	12	12	7	4	2.93	2.719	1.240
27	30	14	12	8	2	4.56	4.577	−0.219
28	35	12	8	9	3	3.05	2.949	0.561
29	35	12	10	8	3	3.78	3.492	1.815
30	35	12	10	7	3	3.35	3.522	−0.705
31	35	12	10	8	3	3.38	3.522	−0.577
32	35	12	10	8	3	3.35	3.522	−0.705
33	35	12	10	8	3	3.38	3.522	−0.577

aTemperature; ^boil concentration; ^cinoculum size; ^dincubation time.