Table 3: Kinetic parameters for purified extracts of the PAL enzyme from Trichosporon cutaneum.

TrialPhenylalaninePhenylalaninePhenylalanineTyrosineTyrosineTyrosinePAL/TAL ratio
( M min−1) (mM) / ( M min−1) (mM) /

1 0.3 0.13.0
2 0.8 0.51.6
3 1.7 0.91.9

For each trial, activity assays for Phe and Tyr were performed on the same enzyme extract. Extracts were obtained from anion exchange chromatography.