Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans
Protein pattern and zymogram of concentrated extracellular extract of recombinant clone expressing NStcI protein in Pichia pastoris. (1) Protein pattern on SDS-PAGE gel after silver staining. M, low molecular weight marker; NStcI, concentrated crude extract of the recombinant clone expressing NStcI protein. (2) Esterase activity on α-NA after renaturation of NStcI protein expressed by recombinant clone. The protein band corresponding to NStcI is indicated with an arrow.