Enzyme Research

Enzyme Research / 2013 / Article / Fig 2

Research Article

Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans

Figure 2

Protein pattern and zymogram of concentrated extracellular extract of recombinant clone expressing NStcI protein in Pichia pastoris. (1) Protein pattern on SDS-PAGE gel after silver staining. M, low molecular weight marker; NStcI, concentrated crude extract of the recombinant clone expressing NStcI protein. (2) Esterase activity on α-NA after renaturation of NStcI protein expressed by recombinant clone. The protein band corresponding to NStcI is indicated with an arrow.