Purification and Characterization of Melanogenic Enzyme Tyrosinase from Button Mushroom

Table 1

Purification of tyrosinase from mushroom A. bisporus.

Fractions	Volume (mL)	Total; protein (mg)	Activity; (units)	Total activity U	Specific activity U/mg	-fold purification	Yield (%)
Crude extract	400	321.4	2.56	1025	3.189	1	100
Ammonium sulphate precipitate	120	77.13	7.13	856	11.09	3.47	83.5
Dialysis	60	33.63	8.25	495	14.71	4.61	48.2
Gel filtration G100 column	30	12.44	12.2	366	29.42	9.22	35.7
DEAE-Cellulose column	15	5.23	18.2	273	52.19	16.36	26.6

DEAE: diethylaminoethyl.