Enzyme Research

Enzyme Research / 2014 / Article / Tab 1

Research Article

Purification and Characterization of Melanogenic Enzyme Tyrosinase from Button Mushroom

Table 1

Purification of tyrosinase from mushroom A. bisporus.

FractionsVolume (mL)Total
protein (mg)
Activity
(units)
Total activity U Specific activity U/mg-fold purification
Yield (%)

Crude extract400321.42.5610253.1891100
Ammonium sulphate precipitate12077.137.1385611.093.4783.5
Dialysis6033.638.2549514.714.6148.2
Gel filtration G100 column3012.4412.236629.429.2235.7
DEAE-Cellulose column155.2318.227352.1916.3626.6

DEAE: diethylaminoethyl.

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