A Fractional Factorial Design to Study the Effect of Process Variables on the Preparation of Hyaluronidase Loaded PLGA Nanoparticles

Figure 2

Contour plot showing the effect of independent variables on size. (a) Effect of various levels of homogenization speed and internal-external phase volume ratio on size when the levels of other variables are maintained at low levels. (b) Effect of various levels of homogenization speed and internal-external phase volume ratio on size when the levels of other variables are maintained at high levels. (c) Effect of various levels of PVA concentration and internal-external phase volume ratio on size when the levels of other variables are maintained at low levels. (d) Effect of various levels of PVA concentration and internal-external phase volume ratio on size when the levels of other variables are maintained at low levels.

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