Research Article
A Fractional Factorial Design to Study the Effect of Process Variables on the Preparation of Hyaluronidase Loaded PLGA Nanoparticles
Table 1
Fractional factorial design.
| Run order | Std order | PLGA () (mg) | PVA () (%w/v) | IEPR () | Speed () (rpm) | Time () (min) | Size (nm) | ZP (mV) | DE (%) |
| 1 | 7 | 50 | 2 | 6 | 7000 | 2 | 630.8 | −28.3 | 22.7 | 2 | 2 | 200 | 0.2 | 2 | 7000 | 2 | 357.3 | −24.1 | 19.64 | 3 | 6 | 200 | 0.2 | 6 | 7000 | 10 | 1058 | −29.8 | 13.58 | 4 | 3 | 50 | 2 | 2 | 7000 | 10 | 1014 | −23.7 | 15.90 | 5 | 4 | 200 | 2 | 2 | 10 000 | 2 | 744.7 | −27.7 | 7.28 | 6 | 5 | 50 | 0.2 | 6 | 10 000 | 2 | 423.8 | −27.1 | 7.65 | 7 | 1 | 50 | 0.2 | 2 | 10 000 | 10 | 329.9 | −22.2 | 4.44 | 8 | 8 | 200 | 2 | 6 | 10 000 | 10 | 387.1 | −38.2 | 10.09 |
| One way ANOVA ( value) | size | 0.87 | 0.51 | 0.95 | 0.17 | 0.49 | | | | ZP | 0.75 | 0.55 | 0.67 | 0.06 | 0.34 | NA | DE | 0.80 | 0.51 | 0.004 | 0.60 | 0.74 | | | |
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Zeta potential; DE: percentage drug encapsulation, IEPR: internal-external phase ratio.
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