A Fractional Factorial Design to Study the Effect of Process Variables on the Preparation of Hyaluronidase Loaded PLGA Nanoparticles

Table 1

Fractional factorial design.

Run order	Std order	PLGA () (mg)	PVA () (%w/v)	IEPR ()	Speed () (rpm)	Time () (min)	Size (nm)	ZP (mV)	DE (%)
1	7	50	2	6	7000	2	630.8	−28.3	22.7
2	2	200	0.2	2	7000	2	357.3	−24.1	19.64
3	6	200	0.2	6	7000	10	1058	−29.8	13.58
4	3	50	2	2	7000	10	1014	−23.7	15.90
5	4	200	2	2	10 000	2	744.7	−27.7	7.28
6	5	50	0.2	6	10 000	2	423.8	−27.1	7.65
7	1	50	0.2	2	10 000	10	329.9	−22.2	4.44
8	8	200	2	6	10 000	10	387.1	−38.2	10.09
One way ANOVA; ( value)	size	0.87	0.51	0.95	0.17	0.49
	ZP	0.75	0.55	0.67	0.06	0.34	NA
	DE	0.80	0.51	0.004	0.60	0.74

Zeta potential; DE: percentage drug encapsulation, IEPR: internal-external phase ratio.