A Fractional Factorial Design to Study the Effect of Process Variables on the Preparation of Hyaluronidase Loaded PLGA Nanoparticles

Table 2

Effect of process variables on size, zeta potential, and drug encapsulation.

Term	Size		Zeta potential		Protein content
	Effect	% cont	Effect	% cont	Effect	% cont
A-PLGA concn	37.15	0.45	−4.63	1.86	1.32	1.21
B-PVA concn	151.9	7.58	−3.68	6.19	−3.32	7.62
C-Int-ext phase ratio	13.45	0.061	−6.43	3.21	−10.59	77.57
D-speed	−293.65	28.34	−2.33	47.30	2.67	4.92
E-time	158.1	8.21	−1.68	15.47	1.69	1.97
BC	−383.85	48.42	−1.13	1.45	3.12	6.71
BE	−145.3	6.94	−1.28	24.51	−0.02	0.0004

% cont: percentage contribution, speed: homogenization speed, time: homogenization time, concn: concentration, BC: interaction between PVA concn and phase ratio, and BE: interaction between PVA concn and time.