Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12
Figure 4
Nucleotide and deduced amino acid sequences of genomic DNA and cDNA encoding protease from G. antarctica strain PI12. The nucleotides are numbered from the 5′ end of the cDNA. A putative CAAT, TATA box, and polyadenylation signal are bold italic. The putative N-terminal signal peptide is underlined. The initiation codon is boldfaced, and termination codon is represented by an asterisk (), boxed peptide sequences showed N-glycosylated asparagines residues. The amino acid residues that form the catalytic triad are double underlined and bold while introns are represented in blue font. Highlighted in grey are the intron donor-acceptor splice sites.
