Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Figure 8

Effect of methanol concentration towards protease activity. The recombinant was cultivated for 72 hours at 30°C in BMMY medium. 0.5% methanol gives the highest activity with 14 U/mL. The substrate (azocasein) was dissolved in Tris HCL buffer and incubated for 30 min at 20°C. The mixture was left for 15 min and absorbance was taken at 450 nm. A one-way ANOVA was used to test for preference differences among six different concentrations. The statistical value (𝑃 value <0.05) between concentration is 0.02.