Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Table 2

Amino acid composition and molecular weight of proteases across different families.

Amino		CAQ76821		CAG25549		EDR11679		CAG38357		CAD11898		CAL25578		EDU50747		AAW40780		ACB30121		ACB30119
acid		Qty	%	Qty	%	Qty	%	Qty	%	Qty	%	Qty	%	Qty	%	Qty	%	Qty	%	Qty	%
Ala	(A)	97	10.1	81	9.1	78	8.7	75	9.0	82	9.9	101	11.5	81	8.9	91	9.6	80	9.2	105	11.6
Arg	(R)	40	4.2	27	3.0	22	2.4	16	1.9	17	2.1	18	2.1	29	3.2	22	2.3	21	2.4	31	3.4
Asn	(N)	44	4.6	38	4.3	43	4.8	41	4.9	42	5.1	40	4.6	52	5.7	51	5.4	45	5.2	43	4.7
Asp	(D)	49	5.1	62	6.9	54	6.0	65	7.8	55	6.7	51	5.8	53	5.8	66	6.9	52	6.0	63	7.0
Cys	(C)	8	0.8	5	0.6	4	0.4	4	0.5	9	1.1	8	0.9	9	1.0	9	0.9	7	0.8	10	1.1
Gln	(Q)	23	2.4	34	3.8	23	2.6	28	3.4	30	3.6	37	4.2	35	3.9	29	3.1	19	2.2	26	2.9
Glu	(E)	21	2.2	16	1.8	19	2.1	20	2.4	15	1.8	20	2.3	25	2.8	26	2.7	28	3.2	24	2.6
Gly	(G)	100	10.4	89	10.0	84	9.3	85	10.2	85	10.3	77	8.8	84	9.3	80	8.4	85	9.8	86	9.5
His	(H)	14	1.5	14	1.6	13	1.4	16	1.9	13	1.6	11	1.3	16	1.8	14	1.5	16	1.8	12	1.3
Ile	(I)	53	5.5	49	5.5	51	5.7	53	6.4	41	5.0	38	4.3	51	5.6	44	4.6	35	4.0	44	4.9
Leu	(L)	95	9.9	68	7.6	71	7.9	62	7.4	60	7.3	66	7.5	67	7.4	80	8.4	74	8.5	68	7.5
Lys	(K)	26	2.7	29	3.2	44	4.9	29	3.5	49	5.9	51	5.8	40	4.4	30	3.2	50	5.8	56	6.2
Met	(M)	12	1.2	2	0.2	2	0.2	9	1.1	11	1.3	8	0.9	18	2.0	8	0.8	11	1.3	15	1.7
Phe	(F)	41	4.3	59	6.6	47	5.2	55	6.6	29	3.5	36	4.1	44	4.9	39	4.1	34	3.9	34	3.8
Pro	(P)	62	6.4	65	7.3	67	7.4	58	7.0	58	7.0	59	6.7	50	5.5	51	5.4	55	6.4	57	6.3
ser	(S)	77	8.0	69	7.7	91	10.1	60	7.2	66	8.0	70	8.0	72	7.9	111	11.7	63	7.3	61	6.7
Thr	(T)	87	9.0	89	10.0	75	8.3	83	10.0	65	7.9	73	8.3	71	7.8	72	7.6	71	8.2	68	7.5
Trp	(W)	5	0.5	3	0.3	6	0.7	2	0.2	12	1.5	12	1.4	12	1.3	9	0.9	12	1.4	14	1.5
Tyr	(Y)	33	3.4	12	1.3	26	2.9	7	0.8	29	3.5	28	3.2	39	4.3	50	5.3	26	3.0	27	3.0
val	(V)	76	7.9	82	9.2	80	8.9	66	7.9	57	6.9	74	8.4	58	6.4	68	7.2	82	9.5	62	6.8
Total		963	100.0	893	100.0	900	100.0	834	100.0	825	100.0	878	100.0	906	100.0	950	100.0	866	100.0	906	100.0
MW	(Da)	100989.8		93266.9		94436.5		86945.5		86954.9		92490.5		97934.3		100962.9		91720.9		96198.8

The accession numbers of proteases: putative serine protease of Glaciozymaantarctica PI12 (CAQ76821), subtilisin-like protease Pleurotus ostreatus (CAG25549), pyrolysin Laccaria bicolor S238N-H82 (EDR11679), subtilisin-like protease Phanerochaete chrysosporium (CAG38357), subtilisin-like serine protease PR1C Metarhizium anisopliae var. anisopliae (CAD11898), serine endopeptidase Hypocrea lixii (CAL25578), peptidase Pyrenophora tritici-repentis (EDU50747), peptidase Cryptococcus neoformans var. neoformans JEC21 (AAW40780), subtilisin-like protease Epichloe festucae (ACB30121), subtilisin-like protease Epichloe festucae (ACB30119).