Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352

Table 1

Different levels of experimental variables used for the production of glucoamylase using Plackett-Burman design.

Symbol	Variable	Units	Coded level
			−1	0	1
𝐴	Soluble starch	(g/L)	5	10	15
𝐵	Yeast extract	(g/L)	1	3	5
𝐶	KH2PO4	(g/L)	0.5	1	1.5
𝐷	K2HPO4	(g/L)	0.5	1	1.5
𝐸	NaCl	(g/L)	0.5	1	1.5
𝐹	CaCl2	(g/L)	0.5	1	1.5
𝐺	MgSO4·7H2O	(g/L)	0.5	1	1.5
𝐻	Vogel’s solution	(mL)	0.1	0.5	1