Enzyme Research

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Research Article

Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352

Table 7

Analysis of variance (ANOVA) for the fitted second-order polynomial model for optimization of glucoamylase production.

VariablesCoefficient estimateSum of squaresDegrees of freedom value value

Model11.6538.95791422.06<0.0001
−0.4862510.0955144.98<0.0001
0.2604175.6745112.90.002
0.2945831.6276116.510.001
−0.1720832.082715.630.03
−0.2298960.1854111.980.003
−0.5486465.5598168.23<0.0001
−0.5986468.3763181.23<0.0001
−0.5686469.2467173.3<0.0001
−0.0668750.071610.570.462
0.0218750.007710.060.809
0.2418750.936117.420.015
0.4456253.1773125.19<0.0001
0.1531250.375212.970.104
−0.2406250.926417.340.015
Residual2.018516
 Lack of fit1.94311015.460.002
 Pure error0.07546

Total40.976330

, , , and PRESS: 11.29.

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