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Enzyme Research
Volume 2014, Article ID 353915, 7 pages
Research Article

Purification and Characterization of a Unique Pectin Lyase from Aspergillus giganteus Able to Release Unsaturated Monogalacturonate during Pectin Degradation

1Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jaú km 1, 14801-902 Araraquara, SP, Brazil
2Department of Biochemistry and Microbiology, Biosciences Institute, Universidade Estadual Paulista (UNESP), Avenida 24A 1515, 13506-900 Rio Claro, SP, Brazil

Received 12 September 2014; Accepted 15 December 2014; Published 31 December 2014

Academic Editor: John David Dignam

Copyright © 2014 Danielle Biscaro Pedrolli and Eleonora Cano Carmona. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A pectin lyase, named PLIII, was purified to homogeneity from the culture filtrate of Aspergillus giganteus grown in submerged culture containing orange peel waste as carbon source. PLIII was able to digest apple pectin and citrus pectins with different degrees of methyl esterification. Interestingly, the PLIII activity was stimulated in the presence of some divalent cations including Pb2+ and was not significantly affected by Hg2+. Like other pectin lyases, PLIII is stimulated by but is not dependent on Ca2+. The main soluble product released during the degradation of pectic substances promoted by the PLIII is compatible with an unsaturated monogalacturonate. PLIII is a unique enzyme able to release unsaturated monogalacturonate as the only soluble product during the degradation of pectic substances; therefore, PLIII was classified as an exo-pectin lyase. To our knowledge, this is the first characterization of an exo-pectin lyase. The PLIII described in this work is potentially useful for ethanol production from pectin-rich biomass, besides other common applications for alkaline pectinases like preparation of textile fibers, coffee and tea fermentation, vegetable oil extraction, and the treatment of pulp in papermaking.