Purification and Characterization of a Unique Pectin Lyase from Aspergillus giganteus Able to Release Unsaturated Monogalacturonate during Pectin Degradation

Figure 5

Degradation pattern of pectic substances by the purified PLIII from A. giganteus. Each lane corresponds to one substrate submitted to enzymatic degradation. Lane 1: digalacturonic acid, lane 2: polygalacturonic acid, lane 3: citrus pectin MD 72%, lane 4: citrus pectin MD 34%, lane 5; apple pectin MD 75%, and lanes 6, 7, and 8; citrus pectin MD 72% after 30 min, 2 h, and 24 h incubation, respectively. Standards: G1: monogalacturonic acid, G2: digalacturonic acid, and G3: trigalacturonic acid. The upper spots in lanes 2, 3, 6, 7, and 8 result from the alkaline hydrolysis of the substrate due to the reaction conditions also seen in the controls without enzyme addition (data not shown).