Purification and Characterization of a Unique Pectin Lyase from Aspergillus giganteus Able to Release Unsaturated Monogalacturonate during Pectin Degradation

Table 1

Pectin lyase purification steps.

Purification step	Total activity (U)	Protein (mg)	Specific activity (U/mg of protein)	Yield (%)	Purification fold
Crude extract	181,300.0	230.9	785.2	100.0	1.0
DEAE-Sephadex A-50	157,529.0	54.3	2,901.1	87.0	3.7
CM-Sephadex C-50
PLI	25,034.4	34.6	724.4	13.8	0.9
PLII	18,012.0	2.5	7,125.0	9.9	9.1
PLIII	52,540.0	1.4	37,528.5	29.0	47.8
Sephadex G-100 (PLIII)	22,500.0	0.6	37,500.0	12.4	47.8