Esterase Active in Polar Organic Solvents from the Yeast Pseudozyma sp. NII 08165

Table 1

Plackett and Burman design matrix for screening for process variables and the responses obtained for different trials.

Std. order	Glucose; (% w/v)	Peptone; (% w/v)	Olive oil; (% v/v)	Inoculum; (% v/v)	pH	Time (h)	(NH4)2SO4; (% w/v)	Esterase (UmL^−1)
1	0.5	0.6	0.1	2	6.5	120	0.4	169
2	1.0	0.6	0.1	1	4.0	120	1.0	1490
3	0.5	1.2	0.1	1	6.5	96	1.0	142
4	1.0	1.2	0.1	2	4.0	96	0.4	2686
5	0.5	0.6	0.3	2	4.0	96	1.0	2777
6	1.0	0.6	0.3	1	6.5	96	0.4	224
7	0.5	1.2	0.3	1	4.0	120	0.4	158
8	1.0	1.2	0.3	2	6.5	120	1.0	219