Contemporaneous Production of Amylase and Protease through CCD Response Surface Methodology by Newly Isolated Bacillus megaterium Strain B69

Table 3

(a) value of the variables for protease and amylase production investigated in the Plackett-Burman design. (b) ANOVA indicating model values for two responses in Placket Burman. (c) Regression values as obtained by ANOVA in Placket Burman.

(a)

Variable	Protease activity				Amylase activity
	Component	+	−		+	−
A	Substrate concentration	1821.66	1271.93	91.62	3752.13	2564.29	197.97
B	Moisture content	1162.03	1931.56	−128.26	2134.36	4182.07	−341.29
C	Inoculum size	1799.08	1294.51	84.09	3527.12	2789.3	122.97
D	Time	2584.46	509.13	345.89	4443.68	1872.75	428.49
E	K2HPO4	1344.81	1748.78	−67.33	3096.21	3220.22	−20.67
F	KH2PO4	1723.75	1369.84	58.99	3651.92	2664.51	164.58
G	NaCl	1715.74	1377.85	56.32	3315.36	3001.07	52.38
H	MgSO4	1507	1586.59	−13.27	2909.39	3407.04	−82.94
I	CaCl2	1565.17	1528.42	6.13	3503.02	2813.4	114.94
J	NaNO3	1358.54	1735.05	−62.75	3002.47	3313.96	−51.91

(b)

Response	Source	Sum of squares	df	Mean square	value	value Prob >
Protease activity	Model		7	70073.44	27.52	0.0032	Significant
Amylase activity	Model		8		45.31	0.0048	Significant

(c)

	Std. Dev.	Adeq precision	-Squared	Adj -Squared	Pred -Squared
Protease activity	50.46	15.365	0.9797	0.9441	0.8169
Amylase activity	57.85	17.662	0.9918	0.9699	0.8687