Contemporaneous Production of Amylase and Protease through CCD Response Surface Methodology by Newly Isolated Bacillus megaterium Strain B69

Table 5

ANOVA for response surface quadratic model.

(a)

Response	1 Protease activity				2 amylase activity				Protease & amylase activity
Source	Sum of squares	Mean square	value	value Prob >	Sum of squares	Mean square	value	-value Prob >	df
Model	2.281E + 006	2.534E + 005	162.08	<0.0001	5.538E + 006	6.154E + 005	33.62	<0.0001	9	Significant
A-subs Conc.	2.441E + 005	2.441E + 005	150.94	<0.0001	2.242E + 005	2.242E + 005	12.25	0.0057	1
B-moisture	88686.05	88686.05	54.83	<0.0001	1.469E + 005	1.469E + 005	8.03	0.0178	1
C-time	1.717E + 005	1.717E + 005	106.17	<0.0001	1.018E + 006	1.018E + 006	55.63	<0.0001	1
	26031.48	26031.48	16.09	0.0025	1506.14	1506.14	0.082	0.7801	1
	4.623E − 003	4.623E − 003	2.858E − 006	0.9987	7648.89	7648.89	0.42	0.5325	1
	31370.31	31370.31	19.39	0.0013	8918.14	8918.14	0.49	0.5011	1
	6.339E + 005	6.339E + 005	391.89	<0.0001	2.331E + 006	2.331E + 006	127.39	<0.0001	1
	91212.08	91212.08	56.39	<0.0001	3.122E + 005	3.122E + 005	17.06	0.0020	1
	1.278E + 006	1.278E + 006	789.83	<0.0001	2.115E + 006	2.115E + 006	115.59	<0.0001	1
Residual	16174.90	1617.49			1.830E + 005	18301.72			10
Lack of fit	13073.08	2614.62	4.21	0.0702	1.365E + 005	27308.68	2.94	0.1310	5	Not significant
Pure error	3101.82	620.36			46473.76	9294.75			5
Cor total	2.345E + 006				5.721E + 006				19

(b)

	Std. dev.	Adeq precision	-squared	Adj -squared	Pred -squared
Protease	39.54	36.329	0.9931	0.9869	0.9551
Amylase	135.28	16.128	0.9680	0.9392	0.8072