Contemporaneous Production of Amylase and Protease through CCD Response Surface Methodology by Newly Isolated Bacillus megaterium Strain B69

Table 6

Validation of the design model.

Run	Factor 1	Factor 2	Factor 3	Response 1 protease activity (U/g)		Response 2 amylase activity (U/g)
	A: substrate concentration (%)	B: moisture ratio	C: time (h)	Experimental	Predicted	Experimental	Predicted
1	10	60	48	618.8	630.57	426.67	451.75
2	30	60	120	742.5	734.02	1104.7	1116.39
3	20	19.77	84	817.9	836.01	1034	1067.11
4	20	45	144.55	626.4	638.06	1012.7	1010.72
5	20	45	84	1286.6	1220.2	1615.4	1627.87