Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study
Figure 1
Hydrodynamic, electrophoretic, and structural characterization of xylanase. (a) Sedimentation coefficient distribution of xylanase. Inset: SDS-PAGE gel followed by glycoproteins or Coomassie staining of xylanase (XYN II) and molecular markers (M). (b) Far-UV circular dichroism spectrum of 0.06–1.1 mg·mL−1 xylanase. Inset: dependence of the molar ellipticity at 220 nm on protein concentration. (c) Plot of with increasing temperature (dotted line). The solid line is a fit of data to (1) and the dashed line was obtained by cooling the unfolded protein after the T ramp.
