Purification and Characterization of Glucose-6-Phosphate Dehydrogenase from Camel Liver

Figure 4

Electrophoretic analysis of (camel liver G6PD) the different purification steps on 7% native polyacrylamide gel. (a) Protein pattern: (1) crude extract, (2) ammonium sulfate fraction, (3) 0.1 M DEAE-cellulose fraction, (4) Sephacryl S-300 purified fraction, and (5) 2^′, 5^′ ADP Sepharose 4B fractions. (b) Enzyme pattern: (1) crude extract, (2) ammonium sulfate fraction, (3) 0.1 M DEAE-cellulose fraction, (4) Sephacryl S-300 purified fraction, and (5) 2^′, 5^′ ADP Sepharose 4B fractions. (c) Subunit molecular weight determination by electrophoretic analysis of camel liver G6PD on 12% SDS-polyacrylamide gel: (1) molecular weight marker proteins and (2) purified camel liver glucose-6-phosphate dehydrogenase enzyme camel liver G6PD. (d) Isoelectrofocusing: (1) isoelectric point (pI) marker proteins and (2) the purified camel liver glucose-6-phosphate dehydrogenase camel liver G6PD.

(a) Protein pattern

(b) Enzyme activity pattern

(c) SDS PAGE

(d) pI