Purification and Characterization of Glucose-6-Phosphate Dehydrogenase from Camel Liver

Table 1

A typical purification scheme of camel liver G6PD.

Purification step	Total protein (mg)	Total activity (unit^†)	Specific activity^‡	Yield; (%)	Fold; purification
Crude extract	156.28	4.4801	0.0286	100	1.00
Ammonium sulphate fraction	49.530	3.8517	0.0777	85.97	2.719
DEAE-cellulose
Camel liver G6PD (0.1 M NaCl)	18.032	3.0527	0.16929	68.13	5.919
Sephacryl S-300
Camel liver G6PD	9.7562	2.5990	0.26647	58.01	9.317
2^′, 5^′ ADP Sepharose 4B
Camel liver G6PD	1.2320	2.2230	1.80438	49.61	63.09

All data is based on 10 gm camel liver.
One unit of glucose-6-phosphate dehydrogenase enzyme activity is identified as the amount of enzyme required to reduce 1 mol of NADP⁺ per minute.
Specific activity is expressed as units/mg protein.