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Enzyme Research
Volume 2014, Article ID 738739, 7 pages
http://dx.doi.org/10.1155/2014/738739
Research Article

Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads

1Department of Chemistry and Environmental Sciences, Universidade Estadual Paulista, IBILCE-UNESP, Rua Cristovão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil
2Department of Pharmacy and Biochemistry, Centro Universitário de Rio Preto, UNIRP, Rua Ivete Gabriel Atique 45, 15025-400 São José do Rio Preto, SP, Brazil

Received 24 January 2014; Revised 24 March 2014; Accepted 24 March 2014; Published 10 April 2014

Academic Editor: Raffaele Porta

Copyright © 2014 Bárbara M. Bonine et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. S. Betigeri and S. H. Neau, “Immobilization of lipase using hydrophilic polymers in the form of hydrogel beads,” Biomaterials, vol. 23, no. 17, pp. 3627–3636, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. A. Telefoncu, E. Dinckaya, and K. D. Vorlop, “Preparation and characterization of pancreatic lipase immobilized in Eudragit-matrix,” Applied Biochemistry and Biotechnology, vol. 26, no. 3, pp. 311–317, 1990. View at Google Scholar · View at Scopus
  3. G. Pencreac'h, M. Leullier, and J. C. Baratti, “Properties of free and immobilized lipase from Pseudomonas cepacia,” Biotechnology Bioengeenering, vol. 57, pp. 181–189, 1997. View at Google Scholar
  4. L. Cao, L. van Langen, and R. A. Sheldon, “Immobilised enzymes: carrier-bound or carrier-free?” Current Opinion in Biotechnology, vol. 14, no. 4, pp. 387–394, 2003. View at Publisher · View at Google Scholar · View at Scopus
  5. C. Spahn and S. D. Minteer, “Enzyme immobilization in biotechnology,” Recent Patents on Engineering, vol. 2, no. 3, pp. 195–200, 2008. View at Publisher · View at Google Scholar · View at Scopus
  6. A. A. Homaei, R. Sariri, F. Vianello, and R. Stevanato, “Enzyme immobilization: an update,” Journal of Chemical Biology, vol. 6, pp. 185–205, 2013. View at Google Scholar
  7. S. Jain, I. Roy, and M. N. Gupta, “A smart bioconjugate of trypsin with alginate,” Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, vol. 32, no. 2, pp. 325–337, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. M. G. Sankalia, R. C. Mashru, J. M. Sankalia, and V. B. Sutariya, “Papain entrapment in alginate beads for stability improvement and site-specific delivery: physicochemical characterization and factorial optimization using neural network modeling,” AAPS PharmSciTech, vol. 6, no. 2, pp. E209–E222, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. T. K. H. Vu and V. V. M. Le, “Biochemical studies on the immobilization of the enzyme invertase (EC.3.2.1.26) in alginate gel and its kinetics,” ASEAN Food Journal, vol. 15, no. 1, pp. 73–78, 2008. View at Google Scholar · View at Scopus
  10. K. Won, S. Kim, K.-J. Kim, H. W. Park, and S.-J. Moon, “Optimization of lipase entrapment in Ca-alginate gel beads,” Process Biochemistry, vol. 40, no. 6, pp. 2149–2154, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. H. Ertesvåg and S. Valla, “Biosynthesis and applications of alginates,” Polymer Degradation and Stability, vol. 59, no. 1–3, pp. 85–91, 1998. View at Google Scholar · View at Scopus
  12. O. Smidsrød and G. Skjak-Braek, “Alginate as immobilization matrix for cells,” Trends in Biotechnology, vol. 8, no. 3, pp. 71–78, 1990. View at Google Scholar · View at Scopus
  13. Á. A. A. de Queiroz, E. D. Passes, S. de Brito Alves, G. S. Silva, O. Z. Higa, and M. Vítolo, “Alginate-poly(vinyl alcohol) core-shell microspheres for lipase immobilization,” Journal of Applied Polymer Science, vol. 102, no. 2, pp. 1553–1560, 2006. View at Publisher · View at Google Scholar · View at Scopus
  14. T. Arakawa and S. N. Timasheff, “Stabilization of protein structure by sugars,” Biochemistry, vol. 21, no. 25, pp. 6536–6544, 1982. View at Google Scholar · View at Scopus
  15. K. Imai, T. Shiomi, K. Uchida, and M. Miya, “Immobilization of enzyme into poly (vinyl alcohol) membrane,” Biotechnology and Bioengineering, vol. 28, no. 11, pp. 1721–1726, 1986. View at Google Scholar · View at Scopus
  16. L. M. O. Arruda and M. Vitolo, “Characterization of invertase entrapped into calcium alginate beads,” Applied Biochemistry and Biotechnology A, vol. 81, no. 1, pp. 23–33, 1999. View at Publisher · View at Google Scholar · View at Scopus
  17. M. A. P. Nunes, H. Vila-Real, P. C. B. Fernandes, and M. H. L. Ribeiro, “Immobilization of naringinase in PVA-alginate matrix using an innovative technique,” Applied Biochemistry and Biotechnology, vol. 160, no. 7, pp. 2129–2147, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. A. Dashevsky, “Protein loss by the microencapsulation of an enzyme (lactase) in alginate beads,” International Journal of Pharmaceutics, vol. 161, no. 1, pp. 1–5, 1998. View at Publisher · View at Google Scholar · View at Scopus
  19. S. Hertzberg, L. Kvittingen, T. Anthonsen, and G. Skjak-Braek, “Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: application in lipase-catalysed reactions,” Enzyme and Microbial Technology, vol. 14, no. 1, pp. 42–47, 1992. View at Publisher · View at Google Scholar · View at Scopus
  20. S. C. Mohapatra and J. T. Hsu, “Optimizing lipase activity, enantioselectivity, and stability with medium engineering and immobilization for beta-blocker synthesis,” Biotechnology Bioengineering, vol. 64, pp. 213–220, 1999. View at Google Scholar
  21. K. Mondal, P. Mehta, B. R. Mehta, D. Varandani, and M. N. Gupta, “A bioconjugate of Pseudomonas cepacia lipase with alginate with enhanced catalytic efficiency,” Biochimica et Biophysica Acta, vol. 1764, no. 6, pp. 1080–1086, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. M. K. Pierozan, E. G. Oestreicher, J. V. Oliveira, D. Oliveira, H. Treichel, and R. L. Cansian, “Studies on immobilization and partial characterization of lipases from wheat seeds (Triticum aestivum),” Applied Biochemistry and Biotechnology, vol. 165, no. 1, pp. 75–86, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. R. T. Patil and T. J. Speake, “Water-based microsphere delivery systems for proteins,” Journal of Pharmaceutical Science, vol. 89, pp. 9–15, 2000. View at Google Scholar
  24. V. N. Enujiugha, F. A. Thani, T. M. Sanni, and R. D. Abigor, “Lipase activity in dormant seeds of the African oil bean (Pentaclethra macrophylla Benth),” Food Chemistry, vol. 88, no. 3, pp. 405–410, 2004. View at Publisher · View at Google Scholar · View at Scopus
  25. B. Rubin and E. A. Dennis, Eds., Lipases: Part A. Biotechnology Methods in Enzymology, Academic Press, New York, NY, USA, 1997.
  26. P. P. Polizelli, F. D. A. Facchini, H. Cabral, and G. O. Bonilla-Rodriguez, “A new lipase isolated from oleaginous seeds from Pachira aquatica (bombacaceae),” Applied Biochemistry and Biotechnology, vol. 150, no. 3, pp. 233–242, 2008. View at Publisher · View at Google Scholar · View at Scopus
  27. P. P. Polizelli, F. D. A. Facchini, and G. O. Bonilla-Rodriguez, “Stability of a Lipase extracted from seeds of Pachira aquatica in commercial detergents and application tests in poultry wastewater pretreatment and fat particle hydrolysis,” Enzyme Research, vol. 2013, Article ID 324061, 6 pages, 2013. View at Publisher · View at Google Scholar
  28. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Google Scholar · View at Scopus
  29. R. C. Chang, S. J. Chou, and J. F. Shaw, “Multiple forms and functions of Candida rugosa lipase,” Biotechnology and Applied Biochemistry, vol. 19, pp. 93–97, 1994. View at Google Scholar
  30. R. Boyer, Modern Experimental Biochemistry, Benjamin Cummings, San Francisco, Calif, USA, 2000.
  31. M. Matsumoto and K. Ohashi, “Effect of immobilization on thermostability of lipase from Candida rugosa,” Biochemical Engineering Journal, vol. 14, no. 1, pp. 75–77, 2003. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Sharma and M. N. Gupta, “Alginate as a macroaffinity ligand and an additive for enhanced activity and thermostability of lipases,” Biotechnology and Applied Biochemistry, vol. 33, no. 3, pp. 161–165, 2001. View at Publisher · View at Google Scholar · View at Scopus
  33. R. Dave and D. Madamwar, “Esterification in organic solvents by lipase immobilized in polymer of PVA-alginate-boric acid,” Process Biochemistry, vol. 41, no. 4, pp. 951–955, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. Z. Knezevic, S. Bobic, A. Milutinovic, B. Obradovic, L. Mojovic, and B. Bugarski, “Alginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane system,” Process Biochemistry, vol. 38, no. 3, pp. 313–318, 2002. View at Publisher · View at Google Scholar · View at Scopus
  35. N. S. Dosanjh and J. Kaur, “Immobilization, stability and esterification studies of a lipase from a Bacillus sp.,” Biotechnology and Applied Biochemistry, vol. 36, no. 1, pp. 7–12, 2002. View at Publisher · View at Google Scholar · View at Scopus
  36. G. D. Altun and S. A. Cetinus, “Immobilization of pepsin on chitosan beads,” Food Chemistry, vol. 100, no. 3, pp. 964–971, 2007. View at Publisher · View at Google Scholar · View at Scopus
  37. E. Santaniello, P. Ferraboschi, and P. Grisenti, “Lipase-catalyzed transesterification in organic solvents: applications to the preparation of enantiomerically pure compounds,” Enzyme and Microbial Technology, vol. 15, no. 5, pp. 367–382, 1993. View at Publisher · View at Google Scholar · View at Scopus