Research Article

A Method for Fast Assessment of OP/CB Exposure in the Japanese Quail (Coturnix coturnix japonica) Using Combined Esterases Enzyme Activity as Biomarkers

Table 1

Rate constant inhibition  min−1 characterization by using different esterase enzyme substrates (AcTChI, BuTChI, PrTChI, and PSA) for blood plasma sample of quail inhibited by CB and OP.

PesticidesAcTChIBuTChIPrTChIPSA

Methomyl
Parathion
Malathion
Trichlorfon

Values in the table are the mean ± SE of three individual quails. The measured data resulted in a calculation over different times (1–60 min) resulting in an inhibition of 83–99% of control activity by inhibition with 4 mM methomyl and 2 mM parathion, malathion, and trichlorfon. Then results were fitted with a single exponential decay using SigmaPlot 11. Significant difference (analysis of variance (ANOVA), ) between malathion with other pesticide compounds (methomyl, parathion and trichlorfon).