A Method for Fast Assessment of OP/CB Exposure in the Japanese Quail (Coturnix coturnix japonica) Using Combined Esterases Enzyme Activity as Biomarkers

Table 2

Half maximal inhibitory concentration (IC₅₀; mM) characterization by using different esterase enzyme substrates (AcTChI, BuTChI, PrTChI, and PSA) for blood plasma sample of quail inhibited by CB and OP compounds.

Pesticides	AcTChI	BuTChI	PrTChI	PSA
Methomyl	0.71±0.05	0.92±0.02	0.86±0.07	0.61±0.02
Parathion	0.12±0.02	0.24±0.05	0.66±0.06	0.22±0.06
Malathion	0.93±0.2^∗	1.11±0.9^∗	1.13±0.2^∗	0.91±0.4^∗
Trichlorfon	0.31±0.03	0.26±0.08	0.7±0.01	0.51±0.09

Values in the table are the mean ± SE of three individual quails. For the measurement of IC₅₀, esterase enzyme was inhibited for 30 min at room temperature 25°C with either 0.25–4 mM CB or 0.125–2 mM OP compounds. Then results were fitted with an exponential decay using SigmaPlot 11. ∗Significant difference (analysis of variance (ANOVA), 𝑃<0.05) between malathion with other pesticide compounds (methomyl, parathion, and trichlorfon).