Research Article
A Method for Fast Assessment of OP/CB Exposure in the Japanese Quail (Coturnix coturnix japonica) Using Combined Esterases Enzyme Activity as Biomarkers
Table 2
Half maximal inhibitory concentration (IC50; mM) characterization by using different esterase enzyme substrates (AcTChI, BuTChI, PrTChI, and PSA) for blood plasma sample of quail inhibited by CB and OP compounds.
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Values in the table are the mean ± SE of three individual quails. For the measurement of IC50, esterase enzyme was inhibited for 30 min at room temperature 25°C with either 0.25–4 mM CB or 0.125–2 mM OP compounds. Then results were fitted with an exponential decay using SigmaPlot 11. Significant difference (analysis of variance (ANOVA), ) between malathion with other pesticide compounds (methomyl, parathion, and trichlorfon). |