Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5

Table 4

Molecular weights and mechanical properties of enzyme degraded P(3HB-co-4HB) copolymer scaffolds.

Polymer	4HB composition (mol%)	10^3 Da		Glass transition temperature; °C ()	Melting temperature; °C ()	Tensile strength (MPa)	Elongation at break (%)
P(3HB) (control)	—	66 ± 6	1.8 ± 0.1	5 ± 1	161 ± 1	—	—
P(3HB-co-16%4HB)	21 ± 2	75 ± 5	2.1 ± 0.3	11 ± 3	156 ± 3	0.31 ± 0.15	48 ± 14
P(3HB-co-29%4HB)	33 ± 4	68 ± 6	1.7 ± 0.2	15 ± 2	154 ± 2	0.12 ± 0.06	7 ± 1
P(3HB-co-45%4HB)	49 ± 2	60 ± 4	1.8 ± 0.2	14 ± 2	151 ± 2	0.32 ± 0.07	30 ± 16
P(3HB-co-63%4HB)	71 ± 3	61 ± 10	2.4 ± 0.3	10 ± 2	136 ± 2	0.42 ± 0.21	15 ± 5
P(3HB-co-91%4HB)	93 ± 1	49 ± 7	1.3 ± 0.7	32 ± 3	45 ± 3	0.25 ± 0.05	31 ± 5

Calculated from GC analysis; changes in 4HB monomer composition of scaffolds subjected to enzymatic degradation.
Calculated from GPC analysis; : number-average molecular weight; : polydispersity index.
Calculated from DSC analysis.
Determined using GoTech A1-3000 Tensile Machine.