Table 1: Fluorometric enzyme assay conditions for recombinant cytochrome P450s (CYPs). 3-Cyano-7-ethoxycoumarin (CEC), 3-cyano-7-hydroxycoumarin (CHC), 7-hydroxycoumarin (HC), 7-benzyloxy-4-(trifluoromethyl)coumarin (BFC), 7-hydroxy-4-(trifluoromethyl)coumarin (HFC), 7-methoxy-4-(trifluoromethyl)coumarin (MFC), excitation wavelength (), and emission wavelength ().

EnzymeEnzyme amount
(pmol)
SubstrateSubstrate concentration (M)Product (nm) (nm)

CYP1A11CEC5CHC410450
CYP1A20.5CEC5CHC410450
CYP2A61Coumarin3HC330460
CYP3A41BFC50HFC409530
CYP19A11.5MFC100HFC409530