Pseudomonas aeruginosa Exopolyphosphatase Is Also a Polyphosphate: ADP Phosphotransferase

Table 2

Kinetic parameters of the full-length paPpx and the N-paPpx variant.

Parameters	Substrate	Hydrolase		Transferase
			N-paPpx		N-paPpx
± SD, μM	polyP75	1.30 ± 0.05	30.67 ± 0.57	nd^2	nd^2
± SD, s^−1	polyP75	57.02 ± 1.20	6.69 ± 0.26	nd^2	nd^2
± SD, s^−1 μM^−1	polyP75	43.84 ± 0.19	0.22 ± 0.10	nd^2	nd^2
± SD, μM	polyP65	3.29 ± 0.19	25.17 ± 0.90	2.49 ± 0.20	3.11 ± 0.10
± SD, s^−1	polyP65	53.03 ± 0.90	6.86 ± 0.17	3.93 ± 0.31	0.29 ± 0.04
± SD, s^−1 μM^−1	polyP65	16.11 ± 0.80	0.27 ± 0.01	1.58 ± 0.13	0.09 ± 0.01
± SD, μM	polyP45	7.14 ± 0.12	23.60 ± 0.50	nd^2	nd^2
± SD, s^−1	polyP45	41.23 ± 0.38	5.29 ± 0.07	nd^2	nd^2
± SD, s^−1 μM^−1	polyP45	5.77 ± 0.15	0.22 ± 0.01	nd^2	nd^2
± SD, μM	polyP25	11.03 ± 0.29	20.83 ± 0.42	3.11 ± 0.1	3.36 ± 0.10
± SD, s^−1	polyP25	40.20 ± 1.34	7.26 ± 0.16	4.28 ± 0.27	0.31 ± 0.02
± SD, s^−1 μM^−1	polyP25	3.64 ± 0.12	0.35 ± 0.01	1.38 ± 0.12	0.09 ± 0.01

Values are means ± SD of at least three experiments performed independently.
¹For determination, paPpx the full-length protein was considered as a dimer and N-paPpx was considered as a monomer.
²nd: not determined.