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Enzyme Research
Volume 2015 (2015), Article ID 573721, 10 pages
http://dx.doi.org/10.1155/2015/573721
Research Article

Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries

1Department of Biological Science, University of State of São Paulo (UNESP), 19806-900 Assis, SP, Brazil
2Food Engineering Faculty, State University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil

Received 3 September 2014; Revised 28 November 2014; Accepted 28 November 2014

Academic Editor: Denise Freire

Copyright © 2015 Douglas Fernandes Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. K. M. Ludwig, P. Oliva-Neto, and D. F. Angelis, “Quantification of Saccharomyces cerevisiae flocculation by bacterial contaminants of alcoholic fermentation,” Ciência e Tecnologia Alimentos, vol. 21, no. 1, pp. 63–68, 2001. View at Google Scholar
  2. P. de Oliva-Neto and F. Yokoya, “Evaluation of bacterial contamination in a fed-batch alcoholic fermentation process,” World Journal of Microbiology and Biotechnology, vol. 10, no. 6, pp. 697–699, 1994. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  3. P. Oliva-Neto, C. Dorta, A. F. A. Carvalho, V. M. G. Lima, and D. F. Silva, “The Brazilian technology of fuel ethanol fermentation—yeast inhibition factors and new perspectives to improve the technology,” in Materials and Processes for Energy: Communicating Current Research and Technological Developments, A. Méndez-Vilas, Ed., vol. 1, pp. 371–379, Formatex, Badajoz, Spain, 1st edition, 2013. View at Google Scholar
  4. I. Migneault, C. Dartiguenave, M. J. Bertrand, and K. C. Waldron, “Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking,” BioTechniques, vol. 37, no. 5, pp. 790–802, 2004. View at Google Scholar · View at Scopus
  5. P. Oliva Neto, K. M. Ludwig, C. Dorta, A. F. A. Carvalho, D. F. Silva, and V. M. G. Lima, “Microbial contamination of the alcholic fermentation for fuel ethanol production,” in Bioenergy: Developing, Research and Inovation, N. Stradioto and E. Lemos, Eds., vol. 1, pp. 407–488, Cultura Acadêmica, São Paulo, Brazil, 2012. View at Google Scholar
  6. M. T. Santos and F. Yokoya, “Characteristics of yeast cell flocculation by Lactobacillus fermentum,” Journal of Fermentation and Bioengineering, vol. 75, no. 2, pp. 151–154, 1993. View at Publisher · View at Google Scholar · View at Scopus
  7. F. Yokoya and P. Oliva-Neto, “Characterization of yeast flocculation by Lactobacillus fermentum,” Revista de Microbiologia, vol. 22, pp. 12–16, 1991. View at Google Scholar
  8. S. H. Hynes, D. M. Kjarsgaard, K. C. Thomas, and W. M. Ingledew, “Use of virginiamycin to control the growth of lactic acid bacteria during alcohol fermentation,” Journal of Industrial Microbiology and Biotechnology, vol. 18, no. 4, pp. 284–291, 1997. View at Publisher · View at Google Scholar · View at Scopus
  9. S. P. Meneghin, F. C. Reis, P. G. De Almeida, and S. R. Ceccato-Antonini, “Chlorine dioxide against bacteria and yeasts from the alcoholic fermentation,” Brazilian Journal of Microbiology, vol. 39, no. 2, pp. 337–343, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. P. de Oliva-Neto and F. Yokoya, “Effect of 3,4,4′-trichlorocarbanilide on growth of lactic acid bacteria contaminants in alcoholic fermentation,” Bioresource Technology, vol. 63, no. 1, pp. 17–21, 1998. View at Publisher · View at Google Scholar · View at Scopus
  11. C. Dorta, P. Oliva-Neto, M. S. de-Abreu-Neto, N. Nicolau-Junior, and A. I. Nagashima, “Synergism among lactic acid, sulfite, pH and ethanol in alcoholic fermentation of Saccharomyces cerevisiae (PE-2 and M-26),” World Journal of Microbiology and Biotechnology, vol. 22, no. 2, pp. 177–182, 2006. View at Publisher · View at Google Scholar · View at Scopus
  12. M. Azarkan, A. El Moussaoui, D. van Wuytswinkel, G. Dehon, and Y. Looze, “Fractionation and purification of the enzymes stored in the latex of Carica papaya,” Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 790, no. 1-2, pp. 229–238, 2003. View at Publisher · View at Google Scholar · View at Scopus
  13. K. M. Ludwig, Flocculation of Saccharomyces cerevisiae—characterization and the action of deflocculation enzymes [Master in Microbiology], Instituto de Biociências, Universidade Estadual Paulista, Rio Claro, Brazil, 1998.
  14. S. Luque, J. M. Benito, and J. Coca, “The importance of specification sheets for pressure-driven membrane processes,” Filtration and Separation, vol. 41, no. 1, pp. 24–28, 2004. View at Publisher · View at Google Scholar · View at Scopus
  15. P. de Oliva-Neto and P. T. P. Menão, “Isomaltulose production from sucrose by Protaminobacter rubrum immobilized in calcium alginate,” Bioresource Technology, vol. 100, no. 18, pp. 4252–4256, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  16. N. Kubota, N. Tatsumoto, T. Sano, and K. Toya, “A simple preparation of half N-acetylated chitosan highly soluble in water and aqueous organic solvents,” Carbohydrate Research, vol. 324, no. 4, pp. 268–274, 2000. View at Publisher · View at Google Scholar · View at Scopus
  17. A. S. Martins, D. N. Peixoto, L. M. C. Paiva, A. D. Panek, and C. L. A. Paiva, “A simple method for obtaining reusable reactors containing immobilized trehalase: characterization of a crude trehalase preparation immobilized on chitin particles,” Enzyme and Microbial Technology, vol. 38, no. 3-4, pp. 486–492, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. A. S. G. Lorenzoni, L. F. Aydos, M. P. Klein, M. A. Z. Ayub, R. C. Rodrigues, and P. F. Hertz, “Continuous production of fructooligosaccharides and invert sugar by chitosan immobilized enzymes: comparison between in fluidized and packed bed reactors,” Journal of Molecular Catalysis B: Enzymatic, 2014. View at Publisher · View at Google Scholar
  19. H. Chen, Q. Zhang, Y. Dang, and G. Shu, “The effect of glutaraldehyde cross-linking on the enzyme activity of immobilized β-galactosidase on chitosan bead,” Advance Journal of Food Science and Technology, vol. 5, no. 7, pp. 932–935, 2013. View at Google Scholar · View at Scopus
  20. G. Spagna, F. Andreani, E. Salatelli, D. Romagnoli, and P. G. Pifferi, “Immobilization of α-L-arabinofuranosidase on chitin and chitosan,” Process Biochemistry, vol. 33, no. 1, pp. 57–62, 1998. View at Publisher · View at Google Scholar · View at Scopus
  21. M. W. Anthonsen, K. M. Vårum, and O. Smidsrød, “Solution properties of chitosans: conformation and chain stiffness of chitosans with different degrees of N-acetylation,” Carbohydrate Polymers, vol. 22, no. 3, pp. 193–201, 1993. View at Publisher · View at Google Scholar · View at Scopus
  22. B. Krajewska, “Chitin and its derivatives as supports for immobilization of enzymes,” Acta Biotechnologica, vol. 11, no. 3, pp. 269–277, 1991. View at Publisher · View at Google Scholar · View at Scopus
  23. R. Laus, M. C. M. Laranjeira, A. O. Martins et al., “Chitosan microspheres crosslinked with tripolyphosphate used for the removal of the acidity, iron (III) and manganese (II) in water contaminated in coal mining,” Química Nova, vol. 29, no. 1, pp. 34–39, 2006. View at Publisher · View at Google Scholar · View at Scopus
  24. M. Koudelka-Hep, N. F. Rooij, and D. J. Strike, “Immobilization of enzymes on microelectrodes using chemical crosslinking,” in Immobilization of Enzimes and Cells, F. G. Bickerstaff, Ed., vol. 10, pp. 83–85, Humana Press, Totowa, NJ, USA, 1997. View at Google Scholar
  25. G. B. Broun, “Chemically aggregated enzymes,” Methods in Enzymology, vol. 44, pp. 263–280, 1976. View at Publisher · View at Google Scholar · View at Scopus
  26. R. Dalla-Vecchia, M. D. G. Nascimento, and V. Soldi, “Synthetic applications of immobilized lipases in polymers,” Química Nova, vol. 27, no. 4, pp. 623–630, 2004. View at Publisher · View at Google Scholar · View at Scopus
  27. T. J. Leighton, R. H. Doi, R. A. J. Warren, and R. A. Kelln, “The relationship of serine protease activity to RNA polymerase modification and sporulation in Bacillus subtilis,” Journal of Molecular Biology, vol. 76, no. 1, pp. 103–122, 1973. View at Publisher · View at Google Scholar · View at Scopus
  28. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Publisher · View at Google Scholar
  29. S. Varavinit, N. Chaokasem, and S. Shobsngob, “Immobilization of a thermostable alpha-amylase,” Science Asia, vol. 28, pp. 247–251, 2002. View at Publisher · View at Google Scholar
  30. A. Johnstone and R. Thorpe, Immunochemistry in Practice, Blackwell Scientific, Oxford, UK, 1987.
  31. F. C. Vasconcellos, G. A. S. Goulart, and M. M. Beppu, “Production and characterization of chitosan microparticles containing papain for controlled release applications,” Powder Technology, vol. 205, no. 1–3, pp. 65–70, 2011. View at Publisher · View at Google Scholar · View at Scopus
  32. R. S. Lima, G. S. Nunes, T. Noguer, and J.-L. Marty, “Enzymatic biosensor for the detection of dithiocarbamate fungicides. Kinetic study of aldehyde dehydrogenase enzyme and biosensor optimization,” Química Nova, vol. 30, no. 1, pp. 9–17, 2007. View at Publisher · View at Google Scholar · View at Scopus
  33. O. Zaborsky, Immobilized Enzymes, CRC Press (Chemical Rubber Co.), Cleveland, Ohio, USA, 1973.
  34. W. K. Chui and L. S. C. Wan, “Prolonged retention of cross-linked trypsin in calcium alginate microspheres,” Journal of Microencapsulation, vol. 14, no. 1, pp. 51–61, 1997. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  35. Y. F. Li, F. Y. Jia, J. R. Li, G. Liu, and Y. Z. Li, “Papain immobilization on a nitrilon fibre carrier containing primary amine groups,” Biotechnology and Applied Biochemistry, vol. 33, no. 1, pp. 29–34, 2001. View at Publisher · View at Google Scholar · View at Scopus
  36. J. W. Gaspari, L. H. Gomes, and F. C. A. Tavares, “Immobilization of inulinase from Kluyveromyces marxianus for the hydrolysis of extracts of Helianthus tuberosus L,” Scientia Agricola, vol. 56, pp. 1135–1140, 1999. View at Google Scholar
  37. L. Qiuhua, M. Jingesu, G. Gangjun, C. Xu, and X. Hong, “Immobilization of Papain on microcrystalline chitosan,” Journal of Branch Campus of the First Military Medical, vol. 1, 1998. View at Google Scholar
  38. L. Hong, W. W. Jun, and X. F. Cai, “Studies on preparation of chitosan microspheres and immobilization of papain,” Journal of South China Agricultural University, vol. 2, 2000. View at Google Scholar
  39. M. Zappino, I. Cacciotti, I. Benucci et al., “Bromelain immobilization on microbial and animal source chitosan films, plasticized with glycerol, for application in wine-like medium: microstructural, mechanical and catalytic characterisations,” Food Hydrocolloids, vol. 45, pp. 41–47, 2015. View at Publisher · View at Google Scholar
  40. E. Taqieddin and M. Amiji, “Enzyme immobilization in novel alginate-chitosan core-shell microcapsules,” Biomaterials, vol. 25, no. 10, pp. 1937–1945, 2004. View at Publisher · View at Google Scholar · View at Scopus
  41. J. M. Guisán, A. Bastida, R. M. Blanco, R. Fernández-Lafuente, and E. García-Junceda, “Immobilization of enzymes on glyoxyl agarose,” in Immobilization of Enzymes and Cells, G. F. Bickerstaff, Ed., pp. 277–287, Humana Press, Totowa, NJ, USA, 1997. View at Google Scholar
  42. M. Paterson, J. M. M. Borba, F. A. D. Melo, and J. I. Moraes, “Evaluating the performance of ethanol fermentation in different situations of industrial process,” Brasil Açucareiro, vol. 106, no. 516, pp. 27–32, 1988. View at Google Scholar
  43. F. E. Alves da Silva, Ethanolic fermentation: influence of sulfuric acid on yeast viability and bacterial and yeast contaminants [M.S. thesis], Instituto de Biociências, Unesp, Rio Claro, Brazil, 1993.
  44. M. H. Otênio, Comparative evaluation of the effect of removal of acid treatment with sulfuric acid in yeast during the recycles in the Bandeirantes Distillery (Brazil) [Master degree], Instituto de Biociências de Rio Claro, Universidade Estadual Paulista, Rio Claro, Brazil, 1998.