Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries
Table 1
Protocols used for papain immobilized on chitin.
Treatment
Immobilization conditions
Pretreatment of support
A
1 g chitin + 1% papaina
—
B
1 g chitin + 1% papain + 0.1% PEI
—
C
1 g chitin + 1% papain + 0.5% PEI
—
D
1 g chitin + 1% papain
2% glutaraldehydeb
E
1 g chitin + 1% papain + 0.1% PEIc
2% glutaraldehyde
F
1 g chitin + 1% papain + 0.5% PEI
2% glutaraldehyde
10 mL of 10 g⋅L−1; b10 mL of 2% (⋅) glutaraldehyde; c0.1 or 0.5% (⋅) in papain solution.
