Enzyme Research

Enzyme Research / 2015 / Article / Tab 2

Research Article

Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries

Table 2

Protocols used for papain immobilized on chitosan.

TreatmentPreparation of chitosan with TPPAdsorption of papain on chitosan with TPP Reaction time
(h)

A1 g chitosan in 100 mL 1% acetic acid solution dripped into 200 mL 1% TPP Washing and addition of the microspheres in 200 mL 1% papain solution (phosphate buffer pH 6.4)5 and 12

B1 g chitosan in 100 mL 1% acetic acid solution dripped into a 200 mL 1% TPP and 1% papain to produce microspheres5 and 12

C1 g chitosan in 100 mL 1% acetic acid solution dripped into a solution of 200 mL 1% TPPAddition of the 1% papain solution instantly after the formation of microspheres 5 and 12

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