Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries

Table 2

Protocols used for papain immobilized on chitosan.

Treatment	Preparation of chitosan with TPP	Adsorption of papain on chitosan with TPP	Reaction time; (h)
A^′	1 g chitosan in 100 mL 1% acetic acid solution dripped into 200 mL 1% TPP	Washing and addition of the microspheres in 200 mL 1% papain solution (phosphate buffer pH 6.4)	5 and 12
B^′	1 g chitosan in 100 mL 1% acetic acid solution dripped into a 200 mL 1% TPP and 1% papain to produce microspheres	—	5 and 12
C^′	1 g chitosan in 100 mL 1% acetic acid solution dripped into a solution of 200 mL 1% TPP	Addition of the 1% papain solution instantly after the formation of microspheres	5 and 12